Context - History

Context - History

PHENOPSIS is a prototype created in 2002 by a close collaboration between LEPSE technicians and the French company OPTIMALOG. It is one of the first platforms for high-throughput phenotyping of plants in a controlled environment (culture chamber).

Since 2004, PHENOPSIS has been used in the GABI-GENOPLANTE project to analyse the variability of leaf growth and transpiration in a natural collection of 24 Arabidopsis thaliana accessions and their response to a range of soil water deficits.

Phenopsis-1_medium

Through this work, an accession with very low sensitivity to water stress was identified (Granier et al., 2006). By decomposing leaf growth into underlying variables such as expansion rate and duration, number and size of epidermal cells in the leaves, compensations between these variables were revealed (Aguirrezabal et al., 2006). PHENOPSIS was also used to analyse the effect of water stress on the cell cycle and endoreduplication process in leaves of transgenic lines under- or over-expressing genes controlling these processes in the DAGOLIGN Research Training Network project (Cookson et al., 2006).

aguirrezabal-2006_reference

For more information, we suggest you read the following publications :

Granier C. et al., (2006) PHENOPSIS, an automated platform for reproducible phenotyping of plant responses to soil water deficit in Arabidopsis thaliana permitted the identification of an accession with low sensitivity to soil water deficit. New Phytologist, 169 (3): 623-635.

Aguirrezabal L. et al., (2006) Plasticity to soil water deficit in Arabidopsis thaliana: dissection of leaf development into underlying growth dynamic and cellular variables reveals invisible phenotypes. Plant, Cell and Environment, 29: 2216-2227.

Cookson SJ, Radziejwoski A, Granier C (2006) Cell and leaf size plasticity in Arabidopsis : what is the role of endoreduplication? Plant, Cell and Environment, 29:1273-1283.